Content deleted Content added
m Task 18 (cosmetic): eval 16 templates: del empty params (1×); |
Citation bot (talk | contribs) Add: doi-access. | Use this bot. Report bugs. | Suggested by Headbomb | Linked from Wikipedia:WikiProject_Academic_Journals/Journals_cited_by_Wikipedia/Sandbox | #UCB_webform_linked 110/438 |
||
| (3 intermediate revisions by 3 users not shown) | |||
Line 1:
{{Short description|Protein-coding gene in the species Homo sapiens}}
{{Infobox_gene}}
'''Golgi reassembly-stacking protein of 55 [[Kilo-|k]][[Unified atomic mass unit|Da]]''' ('''GRASP55''') also known as '''golgi reassembly-stacking protein 2''' ('''GORASP2''') is a [[protein]] that in humans is encoded by the ''GORASP2'' [[gene]].<ref name="pmid10487747">{{cite journal | vauthors = Shorter J, Watson R, Giannakou ME, Clarke M, Warren G, Barr FA | title = GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system | journal = The EMBO Journal | volume = 18 | issue = 18 | pages = 4949–60 | date = September 1999 | pmid = 10487747 | pmc = 1171566 | doi = 10.1093/emboj/18.18.4949 }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: GORASP2 golgi reassembly stacking protein 2, 55kDa | url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=26003 }}</ref> It was identified by its homology with [[GRASP65]] and the [[protein primary structure|protein's amino acid sequence]] was determined by analysis of a [[Molecular cloning|molecular clone]] of its [[complementary DNA]].<ref name=pmid10487747/> The first ([[N-terminus]]) 212 [[Protein structure#Amino acid residues|amino acid residues]] of GRASP55 are highly homologous to those of GRASP65, but the remainder of the 454 amino acid residues are highly diverged from GRASP65.<ref name=pmid10487747/> The conserved region is known as the GRASP domain, and it is conserved among GRASPs of a wide variety of [[eukaryotes]], but not [[plants]].<ref name=entrez/><ref name="zhang2015">{{cite journal | vauthors = Zhang X, Wang Y | title = GRASPs in Golgi Structure and Function | journal = Frontiers in Cell and Developmental Biology | volume = 3 | pages = 84 | year = 2015 | pmid = 26779480 | pmc = 4701983 | doi = 10.3389/fcell.2015.00084 | doi-access = free }}</ref> The [[C-terminus]] portion of the molecule is called the ''SPR domain'' ([[serine]], [[proline]]-rich).<ref name=zhang2015/> GRASP55 is more closely related to homologues in other species, suggesting that GRASP55 is ancestral to GRASP65.<ref name=zhang2015/> GRASP55 is found associated with the [[Golgi apparatus#Structure|medial and trans cisternae]] of the [[Golgi apparatus]].<ref name=zhang2015/>
== Function ==
[[File:CnGRASP55domainsc.jpg|thumb|left|240px|GRASP domain alignment of
GRASP55 is involved in establishing the structure of the Golgi apparatus.<ref name=zhang2015/><ref name="entrez"/> It is a [[peripheral membrane protein]] located on the Golgi [[cisterna]], and it can bind to another GRASP55 located on an adjacent cisterna through the GRASP domain, thus linking the cisternae together through multiple [[protein–protein interaction]]s.<ref name=zhang2015/><ref name="ncbi">{{cite journal|last1=Zhang|first1=Xiaoyan|last2=Wang|first2=Yanzhuang|title=GRASPs in Golgi Structure and Function|journal=Frontiers in Cell and Developmental Biology|volume=3|pages=84|doi=10.3389/fcell.2015.00084|pmid=26779480|pmc=4701983|date=6 January 2016|doi-access=free }}</ref>
GRASP55 is attached to the membrane in two ways; it is [[Myristoylation|myristylated]], which attaches it directly to the [[lipid bilayer]]; it is also bound indirectly by binding to [[BLZF1|golgin-45]], which binds to a [[Rab (G-protein)|Rab protein]], which itself is [[lipidation|lipidated]] and thus anchored to the membrane.<ref name=zhang2015/>
Line 30 ⟶ 31:
* {{cite journal | vauthors = Lane JD, Lucocq J, Pryde J, Barr FA, Woodman PG, Allan VJ, Lowe M | title = Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis | journal = The Journal of Cell Biology | volume = 156 | issue = 3 | pages = 495–509 | date = February 2002 | pmid = 11815631 | pmc = 2173349 | doi = 10.1083/jcb.200110007 }}
* {{cite journal | vauthors = Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP | title = A probability-based approach for high-throughput protein phosphorylation analysis and site localization | journal = Nature Biotechnology | volume = 24 | issue = 10 | pages = 1285–92 | date = October 2006 | pmid = 16964243 | doi = 10.1038/nbt1240 | s2cid = 14294292 }}
* {{cite journal | vauthors = Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M | title = Global, in vivo, and site-specific phosphorylation dynamics in signaling networks | journal = Cell | volume = 127 | issue = 3 | pages = 635–48 | date = November 2006 | pmid = 17081983 | doi = 10.1016/j.cell.2006.09.026 | s2cid = 7827573 | doi-access = free }}
{{refend}}
| |||