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{{Other uses|Hat (disambiguation)}}
[[Image:HAT Selection.png|thumb|300px|right|HAT selection depicted by a [[plasmacytoma]] [[thymidine kinase]] mutant fused with a mortal [[splenic]] [[B-cell]].]]
'''HAT Medium''' ('''[[hypoxanthine]]-[[aminopterin]]-[[thymidine]] medium''') is a selection medium for mammalian cell culture, which relies on the combination of [[aminopterin]], a drug that acts as a powerful [[folate]] metabolism inhibitor by inhibiting [[dihydrofolate reductase]], with [[hypoxanthine]] (a [[purine]] derivative) and [[thymidine]] (a deoxy[[nucleoside]]) which are intermediates in [[DNA synthesis]]. The trick is that aminopterin blocks DNA [[De novo synthesis|''de novo'' synthesis]], which is absolutely required
The enzyme [[dihydrofolate reductase]], which produces [[tetrahydrofolate]] (THF) by the reduction of dihydrofolate, is specifically blocked by aminopterin. THF, acting in association with specific [[protein]]s, can receive single carbon units that are then transferred to specific targets.
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