Sterkiella histriomuscorum: Difference between revisions
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''O. trifallax'' is notable for having as many as 16,000 [[chromosomes]].<ref name=":0" /> For comparison, humans have 46 chromosomes, consisting of 23 [[diploid|pairs]]. |
''O. trifallax'' is notable for having as many as 16,000 [[chromosomes]].<ref name=":0" /> For comparison, humans have 46 chromosomes, consisting of 23 [[diploid|pairs]]. |
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== Genomics == |
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The draft macronuclear [[genome]] of ''O. trifallax'' was published in 2013.<ref name=":0">[http://www.plosbiology.org/article/info:doi%2F10.1371%2Fjournal.pbio.1001473 The Oxytricha trifallax Macronuclear Genome: A Complex Eukaryotic Genome with 16,000 Tiny Chromosomes.]</ref> |
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In ''O. trifallax'', the [[telomere|telomeric]] repeats (5' to 3' toward the end) are formed by the known nucleotide sequence TTTTGGGG. |
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The development of the <span>of the </span>''Oxytricha''<span> macronuclear genome from the micronuclear genome is as follows: </span> |
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[[File:Development of the Oxytricha macronuclear genome.jpg|thumb|left|Development of the ''Oxytricha'' macronuclear genome from the micronuclear genome.|281x281px]] |
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During conjugation of ''Oxytricha'' cells, segments of the micronuclear genome (MDSs) are excised and stitched together to form the nanochromosomes of the new macronuclear genome, and the remainder of the micronuclear genome is eliminated (including the IESs interspersed between MDSs). The old macronuclear genome is also degraded during development. The segments that are stitched together may be either in order (e.g., forming nanochromosome 1) or out of order or inverted (e.g., forming the two forms of nanochromosome 2), in which case they need to be “unscrambled.” |
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Two rounds of DNA amplification produce nanochromosomes at an average copy number of ~1,900. Alternative fragmentation of DNA during nanochromosome development may also occur, irrespective of unscrambling, giving rise to longer (2a) and shorter (2b) nanochromosome isoforms. The mature nanochromosomes are capped on both ends with telomeres. |
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== References == |
== References == |
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Revision as of 06:50, 25 August 2014
| Oxytricha trifallax | |
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| Oxytricha trifallax | |
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| Species: | O. trifallax
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| Oxytricha trifallax Stein, 1859
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Oxytricha trifallax is a ciliate protozoan species in the genus Oxytricha.
O. trifallax is notable for having as many as 16,000 chromosomes.[1] For comparison, humans have 46 chromosomes, consisting of 23 pairs.
hei på dei!!!
References
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