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Sporopollenin

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SEM image of pollen grains

Sporopollenin is a biological polymer found as a major component of the tough outer (exine) walls of plant spores and pollen grains. It is chemically very stable (one of the most inert among biopolymers)[1] and is usually well preserved in soils and sediments. The exine layer is often intricately sculptured in species-specific patterns, allowing material recovered from (for example) lake sediments to provide useful information to palynologists about plant and fungal populations in the past. Sporopollenin has found uses in the field of paleoclimatology as well. Sporopollenin is also found in the cell walls of several taxa of green alga, including Phycopeltis (an ulvophycean)[2] and Chlorella.[3]

Spores are dispersed by many different environmental factors, such as wind, water or animals. In suitable conditions, the sporopollenin-rich walls of pollen grains and spores can persist in the fossil record for hundreds of millions of years, since sporopollenin is resistant to chemical degradation by organic and inorganic chemicals.[4]

Chemical composition

The chemical composition of sporopollenin has long been elusive due to its unusual chemical stability, insolubility and resistance to degradation by enzymes and strong chemical reagents. It was once thought to consist of polymerised carotenoids but the application of more detailed analytical methods since the 1980s has shown that this is not correct.[5] Analyses have revealed a complex biopolymer, containing mainly long-chain fatty acids, phenylpropanoids, phenolics and traces of carotenoids in a random co-polymer. It is likely that sporopollenin derives from several precursors that are chemically cross-linked to form a rigid structure.[4] There is also good evidence that the chemical composition of sporopollenin is not the same in all plants, indicating it is a class of compounds rather than having one constant structure.[5]

In 2019, thioacidolysis degradation and solid-state NMR was used to determine the molecular structure of pitch pine sporopollenin, finding it primarily composed of polyvinyl alcohol units alongside other aliphatic monomers, all crosslinked through a series of acetal linkages. Its complex and heterogeneous chemical structure give some protection from the biodegradative enzymes of bacteria, fungi and animals.[6] Some aromatic structures based on p-coumarate and naringenin were also identified within the sporopollenin polymer. These can absorb ultraviolet light and thus prevent it penetrating further into the spore. This has relevance to the role of pollen and spores in transporting and dispersing the gametes of plants. The DNA of the gametes is readily damaged by the ultraviolet component of daylight. Sporopollenin thus provides some protection from this damage as well as a physically robust container.[6]

Analysis of sporopollenin from the clubmoss Lycopodium in the late 1980s have shown distinct structural differences from that of flowering plants.[5] In 2020, more detailed analysis of sporopollenin from Lycopodium clavatum provided more structural information. It showed a complete lack of aromatic structures and the presence of a macrocyclic backbone of polyhydroxylated tetraketide-like monomers with pseudo-aromatic 2-pyrone rings. These were crosslinked to a poly(hydroxy acid) chain by ether linkages to form the polymer.[7]

Biosynthesis

Electron microscopy shows that the tapetal cells that surround the developing pollen grain in the anther have a highly active secretory system containing lipophilic globules.[8] These globules are believed to contain sporopollenin precursors. Tracer experiments have shown that phenylalanine is a major precursor, but other carbon sources also contribute.[4] The biosynthetic pathway for phenylpropanoid is very active in tapetal cells, supporting the idea that its products are needed for sporopollenin synthesis. Chemical inhibitors of pollen development and many male sterile mutants have effects on the secretion of these globules by the tapetal cells.[8]

See also

References

  1. ^ The Evolution of Plant Physiology. London: Elsevier Academic Press. 2004-02-05. p. 45. ISBN 978-0-12-339552-8.
  2. ^ Good, B. H.; Chapman, R. L. (1978). "The Ultrastructure of Phycopeltis (Chroolepidaceae: Chlorophyta). I. Sporopollenin in the Cell Walls". American Journal of Botany. 65 (1): 27–33. doi:10.2307/2442549. JSTOR 2442549.
  3. ^ Atkinson, A. W.; Gunning, B. E. S.; John, P. C. L. (1972). "Sporopollenin in the cell wall of Chlorella and other algae: Ultrastructure, chemistry, and incorporation of 14C-acetate, studied in synchronous cultures". Planta. 107 (1): 1–32. Bibcode:1972Plant.107....1A. doi:10.1007/BF00398011. PMID 24477346. S2CID 19630391.
  4. ^ a b c Shaw, G. (1971), "THE CHEMISTRY OF SPOROPOLLENIN", Sporopollenin, Elsevier, pp. 305–350, doi:10.1016/b978-0-12-135750-4.50017-1, ISBN 9780121357504
  5. ^ a b c Guilford, W. J.; Opella, S. J.; Schneider, D. M.; Labovitz, J. (1988). "High Resolution Solid State 13C NMR Spectroscopy of Sporopollenins from Different Plant Taxa". Plant Physiology. 86 (1): 134–136. doi:10.1104/pp.86.1.134. JSTOR 4271095. PMC 1054442. PMID 16665854.
  6. ^ a b Weng, Jing-Ke; Hong, Mei; Jacobowitz, Joseph; Phyo, Pyae; Li, Fu-Shuang (January 2019). "The molecular structure of plant sporopollenin". Nature Plants. 5 (1): 41–46. Bibcode:2019NatPl...5...41L. doi:10.1038/s41477-018-0330-7. ISSN 2055-0278. OSTI 1617031. PMID 30559416. S2CID 56174700.
  7. ^ Mikhael, Abanoub; Jurcic, Kristina; Schneider, Celine; others, and 7 (2020). "Demystifying and unravelling the molecular structure of the biopolymer sporopollenin". Rapid Communications in Mass Spectrometry. 34 (10): e8740. Bibcode:2020RCMS...34.8740M. doi:10.1002/rcm.8740. PMID 32003875. S2CID 210984485. Retrieved 8 July 2021.{{cite journal}}: CS1 maint: numeric names: authors list (link)
  8. ^ a b Boavida, L. C.; Becker, J. D.; Feijo, J. A. (2005). "The making of gametes in higher plants". The International Journal of Developmental Biology. 49 (5–6): 595–614. doi:10.1387/ijdb.052019lb. hdl:10400.7/77. PMID 16096968.

Further reading

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