3-Alfa,7-alfa,12-alfa-trihidroksi-5-beta-holestanoil-KoA 24-hidroksilaza
(Preusmjereno sa stranice THCA-CoA oxidase)
3-Alfa,7-alfa,12-alfa-trihidroksi-5-beta-holestanoil-KoA 24-hidroksilaza (EC 1.17.99.3, trihidroksikoprostanoil-KoA oksidaza, THC-KoA oksidaza, THCA-KoA oksidaza, 3alfa,7alfa,12alfa-trihidroksi-5beta-holestanoil-KoA oksidaza, 3alfa,7alfa,12alfa-trihidroksi-5beta-holestan-26-oat 24-hidroksilaza) je enzim sa sistematskim imenom (25R)-3alfa,7alfa,12alfa-trihidroksi-5beta-holestan-26-oil-KoA:akceptor 24-oksidoreduktaza (24R-hidroksilacija).[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 3-Alfa,7-alfa,12-alfa-trihidroksi-5-beta-holestanoil-KoA 24-hidroksilaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.17.99.3 | ||||||||
| CAS broj | 119799-47-2 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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- (25R)-3alfa,7alfa,12alfa-trihidroksi-5beta-holestan-26-oil-KoA + H2O + akceptor (24R,25R)-3alfa,7alfa,12alfa,24-tetrahidroksi-5beta-holestan-26-oil-KoA + redukovani akceptor
Za dejstvo ovog enzima je neophodan ATP. Reakcija kod sisara verovatno obuhvata dehidrogenaciju čime se formira 24(25)-dvostruka veza, čemu sledi hidratacija.
Reference
uredi- ↑ Gustafsson, J. (1975). „Biosynthesis of cholic acid in rat liver. 24-Hydroxylation of 3α,7α,12α-trihydroxy-5β-cholestanoic acid”. J. Biol. Chem. 250: 8243-8247. PMID 240854.
- ↑ Schepers, L., Van Veldhoven, P.P., Casteels, M., Eyssen, H.J. and Mannaerts, G.P. (1990). „Presence of three acyl-CoA oxidases in rat liver peroxisomes. An inducible fatty acyl-CoA oxidase, a noninducible fatty acyl-CoA oxidase, and a noninducible trihydroxycoprostanoyl-CoA oxidase”. J. Biol. Chem. 265: 5242-5246. PMID 2156865.
- ↑ Dieuaide-Noubhani, M., Novikov, D., Baumgart, E., Vanhooren, J.C., Fransen, M., Goethals, M., Vandekerckhove, J., Van Veldhoven, P.P. and Mannaerts, G.P. (1996). „Further characterization of the peroxisomal 3-hydroxyacyl-CoA dehydrogenases from rat liver. Relationship between the different dehydrogenases and evidence that fatty acids and the C27 bile acids di- and tri-hydroxycoprostanic acids are metabolized by separate multifunctional proteins”. Eur. J. Biochem. 240: 660-666. PMID 8856068.
- ↑ Dieuaide-Noubhani, M., Novikov, D., Baumgart, E., Vanhooren, J.C., Fransen, M., Goethals, M., Vandekerckhove, J., Van Veldhoven, P.P. and Mannaerts, G.P. (1997). „Erratum report. Further characterization of the peroxisomal 3-hydroxyacyl-CoA dehydrogenases from rat liver. Relationship between the different dehydrogenases and evidence that fatty acids and the C27 bile acids di- and tri-hydroxycoprostanic acids are metabolized by separate multifunctional proteins”. Eur. J. Biochem. 243: 537-537. PMID 8856068.
- ↑ Pedersen, J.I., Eggertsen, G., Hellman, U., Andersson, U. and Björkhem, I. (1997). „Molecular cloning and expression of cDNA encoding 3α,7α,12α-trihydroxy-5β-cholestanoyl-CoA oxidase from rabbit liver”. J. Biol. Chem. 272: 18481-18489. PMID 9218493.
- ↑ Russell, D.W. (2003). „The enzymes, regulation, and genetics of bile acid synthesis”. Annu. Rev. Biochem. 72: 137-174. PMID 12543708.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.