Ramnogalakturonanska hidrolaza
(Preusmjereno sa stranice RGaza A)
| Ramnogalakturonanska hidrolaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.2.1.171 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Ramnogalakturonanska hidrolaza (EC 3.2.1.171, ramnogalakturonaza A, RGaza A, RG-hidrolaza) je enzim sa sistematskim imenom ramnogalakturonan alfa-D-GalA-(1->2)-alfa-L-Rha hidrolaza.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Endohidroliza alfa-D-GalA-(1->2)-alfa-L-Rha glikozidne veze u ramnogalakturonan I osnovi sa inicijalnom inverzijom anomerne konfiguracije čime se odvajaju oligosaharidi sa beta-D-GalA ostatkom na redukujućem kraju
Ovaj enzim učestvuje u degradaciji ramnogalakturonana I kod Aspergillus aculeatus.
- ↑ Petersen, T.N., Kauppinen, S. and Larsen, S. (1997). „The crystal structure of rhamnogalacturonase A from Aspergillus aculeatus: a right-handed parallel β helix”. Structure 5: 533-544. PMID 9115442.
- ↑ Kofod, L.V., Kauppinen, S., Christgau, S., Andersen, L.N., Heldt-Hansen, H.P., Dorreich, K. and Dalboge, H. (1994). „Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus”. J. Biol. Chem. 269: 29182-29189. PMID 7961884.
- ↑ Azadi, P., O'Neill, M.A., Bergmann, C., Darvill, A.G. and Albersheim, P. (1995). „The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase”. Glycobiology 5: 783-789. PMID 8720076.
- ↑ Petersen, T.N., Christgau, S., Kofod, L.V., Kauppinen, S., Johnson, A.H. and Larsen, S. (1997). „Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus”. Acta Crystallogr. D Biol. Crystallogr. 53: 105-107. PMID 15299976.
- ↑ Pitson, S.M., Mutter, M., van den Broek, L.A., Voragen, A.G. and Beldman, G. (1998). „Stereochemical course of hydrolysis catalysed by α-L-rhamnosyl and α-D-galacturonosyl hydrolases from Aspergillus aculeatus”. Biochem. Biophys. Res. Commun. 242: 552-559. PMID 9464254.
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