Krieg has an eye patch under his mask when it's intact, but once his mask is broken the eye patch is gone and there doesn't appear to be anything wrong with his right eye.
The animated pictures throughout the movie are see-through and show what they're showing from the front and the back. When Lilith finds the picture of her and her mother, it can only be seen from the front and is black from the back.
Lilith is 22 years old in the first Borderlands game but is played by a 55 year old Cate Blanchett.
During Lilith's and Tina's talk in the rooftop, right before Tina starts crying, the colour of the band-aid on her nose changes from pink to nude, then back again. This also happens a few times during the final battle.
It's determined in the Caustic Caverns fight that Claptrap can't use stairs, as Krieg had to drag him to the top, yet when Lilith goes up the stairs into her old house claptrap manages to joins her inside without any assistance.
During the ending, when Tina is throwing grenades around, 3 atlas soldiers stand in a scene, and then blow up. The soldier in the middle before the explosion happens, stands very casual and rather careless in his body language unlike the two other soldiers who look like they are watching towards Tina.
Claptrap's wheel creates a small cloud from the dirt and dust it tosses up but doesn't leave any tire tracks.