SFRS14
Pretpostavljeni faktor prerade RNK, arginin/serinom bogati protein 14 je protein koji je kod ljudi kodiran genom SFRS14.[5][6]
Aminokiselinska sekvenca
[uredi | uredi izvor]- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MAARRITQET | FDAVLQEKAK | RYHMDASGEA | VSETLQFKAQ | DLLRAVPRSR | ||||
| AEMYDDVHSD | GRYSLSGSVA | HSRDAGREGL | RSDVFPGPSF | RSSNPSISDD | ||||
| SYFRKECGRD | LEFSHSDSRD | QVIGHRKLGH | FRSQDWKFAL | RGSWEQDFGH | ||||
| PVSQESSWSQ | EYSFGPSAVL | GDFGSSRLIE | KECLEKESRD | YDVDHPGEAD | ||||
| SVLRGGSQVQ | ARGRALNIVD | QEGSLLGKGE | TQGLLTAKGG | VGKLVTLRNV | ||||
| STKKIPTVNR | ITPKTQGTNQ | IQKNTPSPDV | TLGTNPGTED | IQFPIQKIPL | ||||
| GLDLKNLRLP | RRKMSFDIID | KSDVFSRFGI | EIIKWAGFHT | IKDDIKFSQL | ||||
| FQTLFELETE | TCAKMLASFK | CSLKPEHRDF | CFFTIKFLKH | SALKTPRVDN | ||||
| EFLNMLLDKG | AVKTKNCFFE | IIKPFDKYIM | RLQDRLLKSV | TPLLMACNAY | ||||
| ELSVKMKTLS | NPLDLALALE | TTNSLCRKSL | ALLGQTFSLA | SSFRQEKILE | ||||
| AVGLQDIAPS | PAAFPNFEDS | TLFGREYIDH | LKAWLVSSGC | PLQVKKAEPE | ||||
| PMREEEKMIP | PTKPEIQAKA | PSSLSDAVPQ | RADHRVVGTI | DQLVKRVIEG | ||||
| SLSPKERTLL | KEDPAYWFLS | DENSLEYKYY | KLKLAEMQRM | SENLRGADQK | ||||
| PTSADCAVRA | MLYSRAVRNL | KKKLLPWQRR | GLLRAQGLRG | WKARRATTGT | ||||
| QTLLSSGTRL | KHHGRQAPGL | SQAKPSLPDR | NDAAKDCPPD | PVGPSPQDPS | ||||
| LEASGPSPKP | AGVDISEAPQ | TSSPCPSADI | DMKTMETAEK | LARFVAQVGP | ||||
| EIEQFSIENS | TDNPDLWFLH | DQNSSAFKFY | RKKVFELCPS | ICFTSSPHNL | ||||
| HTGGGDTTGS | QESPVDLMEG | EAEFEDEPPP | REAELESPEV | MPEEEDEDDE | ||||
| DGGEEAPAPG | GAGKSEGSTP | ADGLPGEAAE | DDLAGAPALS | QASSGTCFPR | ||||
| KRISSKSLKV | GMIPAPKRVC | LIQEPKVHEP | VRIAYDRPRG | RPMSKKKKPK | ||||
| DLDFAQQKLT | DKNLGFQMLQ | KMGWKEGHGL | GSLGKGIREP | VSVGTPSEGE | ||||
| GLGADGQEHK | EDTFDVFRQR | MMQMYRHKRA | NK |
Kloniranje i ekspresija
[uredi | uredi izvor]Sekvenciranjem nasumično odabranih cDNK, koje odgovaraju relativno dugim transkriptima iz ljudskog mozga, Nagase et al. (1997) identificirali su djelimičnu SFRS14 cDNK, koju su nazvali KIAA0365.
Pretragom baze podataka EST i analizom genomskih sekvenci, Sampson i Hewitt (2003) identificirali su SFRS14 u punoj dužini, kao i povezanog člana porodice SURP, SF4 (SUGP1). SFRS14 kodira izvedeni protein od 1,083 aminokiseline, s procijenjenom molekulskom masom od 120,2 kD. I SF4 i SFRS14 sadrže 2 SURP motiv i jedna domen G-plohe na svojim C-krajevima. SFRS14 također sadrži N-kraj bogat argininima /serinima, što sugerira da je novi član porodice pre-iRNK faktora prerade povezanih sa SR.
Sampson i Hewitt (2003) također su klonirali mišje ortologe SF4 i SFRS14. Mišji protein Sfrs14 ima identičnu organizaciju domena i 73% identiteta sekvence sa svojim ljudskim homologom. Northern blot analiza otkrila je ekspresiju otprilike 3,5 kb SFRS14 transkripta samo u sjemenicima i fetusnom mozgu i bubrezima. RT-PCR identificirao je ekspresiju SFRS14 u skeletnim mišićima i mozgu odraslih.
Funkcija gena
[uredi | uredi izvor]Na temelju prisutnosti domena SURP, G-plohe i RS, Sampson i Hewitt (2003) sugeriraju da SFRS14 funkcionira u mehanizmima prerade preiRNK.
Struktura gena
[uredi | uredi izvor]Sampson i Hewitt (2003) utvrdili su da gen SFRS14 sadrži 12 egzona i da se proteže na 41,2 kb. SFRS14 podvrgava se [[alternativna prerada RNK|alternativnoj 3-osnovnoj preradi. Najvećem SFRS14 transkriptu nedostaje egzon 11, koji čini prva 103 bazna para UTR-a s tri prajmera.
Mapiranje
[uredi | uredi izvor]Hibridnom analizom zračenja Nagase et al. (1997) mapirali su gen SFRS14 na hromosomu 19. Analizom sekvence, Sampson i Hewitt (2003) mapirali su gene SFRS14 i SF4 unutar 300 kb, jedan od drugog, na hromosomu 19, pozicija na kratkom kraku: p13.11.[7][8]
Reference
[uredi | uredi izvor]- ^ a b c GRCh38: Ensembl release 89: ENSG00000064607 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000036054 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Sampson ND, Hewitt JE (Feb 2003). "SF4 and SFRS14, two related putative splicing factors on human chromosome 19p13.11". Gene. 305 (1): 91–100. doi:10.1016/S0378-1119(02)01230-1. PMID 12594045.
- ^ "Entrez Gene: SFRS14 splicing factor, arginine/serine-rich 14".
- ^ Nagase, T., Ishikawa, K., Nakajima, D., Ohira, M., Seki, N., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 4: 141-150, 1997. [PubMed]: 9205841
- ^ Sampson, N. D., Hewitt, J. E. SF4 and SFRS14, two related putative splicing factors on human chromosome 19p13.11. Gene 305: 91-100, 2003. [PubMed]: 12594045
Dopunska literatura
[uredi | uredi izvor]- Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569. S2CID 13709685.
- Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
- Grimwood J, Gordon LA, Olsen A, et al. (2004). "The DNA sequence and biology of human chromosome 19". Nature. 428 (6982): 529–35. doi:10.1038/nature02399. PMID 15057824.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Nagase T, Ishikawa K, Nakajima D, et al. (1997). "Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 4 (2): 141–50. doi:10.1093/dnares/4.2.141. PMID 9205841.
Vanjski linkovi
[uredi | uredi izvor]- SUGP2 lokacija ljudskog genoma UCSC Genome Browser.
SUGP2 detalji ljudskog genoma u UCSC Genome Browser.