LIMK1

LIM domain kinase 1 is an enzyme that in humans is encoded by the LIMK1 gene.

LIMK1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3S95, 5L6W, 5HVK, 5HVJ
Identifiers
Aliases	LIMK1, LIMK, LIMK-1, LIM domain kinase 1
External IDs	OMIM: 601329; MGI: 104572; HomoloGene: 1738; GeneCards: LIMK1; OMA:LIMK1 - orthologs
Gene location (Human) Chr. Chromosome 7 (human) Band 7q11.23 Start 74,082,933 bp End 74,122,525 bp
Gene location (Mouse) Chr. Chromosome 5 (mouse) Band 5 74.73 cM|5 G2 Start 134,684,893 bp End 134,717,452 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in stromal cell of endometrium right frontal lobe cingulate gyrus anterior cingulate cortex prefrontal cortex amygdala Brodmann area 9 granulocyte ganglionic eminence monocyte Top expressed in motor neuron gastrula internal carotid artery decidua substantia nigra external carotid artery pontine nuclei medial vestibular nucleus anterior horn of spinal cord supraoptic nucleus More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity nucleotide binding heat shock protein binding metal ion binding kinase activity protein binding protein heterodimerization activity ATP binding protein kinase activity protein serine/threonine kinase activity signal transducer activity Cellular component membrane focal adhesion cytoplasm nucleus Golgi apparatus cytosol nuclear speck neuron projection lamellipodium cell projection Biological process Fc-gamma receptor signaling pathway involved in phagocytosis phosphorylation nervous system development negative regulation of ubiquitin-protein transferase activity Rho protein signal transduction positive regulation of actin filament bundle assembly actin cytoskeleton organization signal transduction positive regulation of axon extension protein phosphorylation positive regulation of stress fiber assembly Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 3984 16885 Ensembl ENSG00000106683 ENSMUSG00000029674 UniProt P53667 P53668 RefSeq (mRNA) NM_016735 NM_001204426 NM_002314 NM_010717 NM_001305875 RefSeq (protein) NP_001191355 NP_002305 NP_001292804 NP_034847 Location (UCSC) Chr 7: 74.08 – 74.12 Mb Chr 5: 134.68 – 134.72 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Function

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs, a central PDZ domain, and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development.

Clinical significance

LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.

Interactions

LIMK1 has been shown to interact with:

• CFL1,
• CDKN1C,
• NRG1,
• PAK1,
• PAK4, and
• YWHAZ.

Further reading

• Scott RW, Olson MF (2007). "LIM kinases: function, regulation and association with human disease". J. Mol. Med. 85 (6): 555–68. doi:10.1007/s00109-007-0165-6. PMID 17294230. S2CID 8881246.
• Mizuno K, Okano I, Ohashi K, Nunoue K, Kuma K, Miyata T, Nakamura T (1994). "Identification of a human cDNA encoding a novel protein kinase with two repeats of the LIM/double zinc finger motif". Oncogene. 9 (6): 1605–12. PMID 8183554.
• Okano I, Hiraoka J, Otera H, Nunoue K, Ohashi K, Iwashita S, Hirai M, Mizuno K (1995). "Identification and characterization of a novel family of serine/threonine kinases containing two N-terminal LIM motifs". J. Biol. Chem. 270 (52): 31321–30. doi:10.1074/jbc.270.52.31321. PMID 8537403.
• Frangiskakis JM, Ewart AK, Morris CA, Mervis CB, Bertrand J, Robinson BF, Klein BP, Ensing GJ, Everett LA, Green ED, Pröschel C, Gutowski NJ, Noble M, Atkinson DL, Odelberg SJ, Keating MT (1996). "LIM-kinase1 hemizygosity implicated in impaired visuospatial constructive cognition". Cell. 86 (1): 59–69. doi:10.1016/S0092-8674(00)80077-X. PMID 8689688. S2CID 17786888.
• Nebl G, Meuer SC, Samstag Y (1996). "Dephosphorylation of serine 3 regulates nuclear translocation of cofilin". J. Biol. Chem. 271 (42): 26276–80. doi:10.1074/jbc.271.42.26276. PMID 8824278.
• Hiraoka J, Okano I, Higuchi O, Yang N, Mizuno K (1996). "Self-association of LIM-kinase 1 mediated by the interaction between an N-terminal LIM domain and a C-terminal kinase domain". FEBS Lett. 399 (1–2): 117–21. doi:10.1016/S0014-5793(96)01303-8. PMID 8980133. S2CID 2815035.
• Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E, Mizuno K (1998). "Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization". Nature. 393 (6687): 809–12. Bibcode:1998Natur.393..809Y. doi:10.1038/31735. PMID 9655398. S2CID 4326365.
• Wang JY, Frenzel KE, Wen D, Falls DL (1998). "Transmembrane neuregulins interact with LIM kinase 1, a cytoplasmic protein kinase implicated in development of visuospatial cognition". J. Biol. Chem. 273 (32): 20525–34. doi:10.1074/jbc.273.32.20525. PMID 9685409.
• Edwards DC, Gill GN (1999). "Structural features of LIM kinase that control effects on the actin cytoskeleton". J. Biol. Chem. 274 (16): 11352–61. doi:10.1074/jbc.274.16.11352. PMID 10196227.
• Sotiropoulos A, Gineitis D, Copeland J, Treisman R (1999). "Signal-regulated activation of serum response factor is mediated by changes in actin dynamics". Cell. 98 (2): 159–69. doi:10.1016/S0092-8674(00)81011-9. PMID 10428028. S2CID 15781260.
• Bach I, Rodriguez-Esteban C, Carrière C, Bhushan A, Krones A, Rose DW, Glass CK, Andersen B, Izpisúa Belmonte JC, Rosenfeld MG (1999). "RLIM inhibits functional activity of LIM homeodomain transcription factors via recruitment of the histone deacetylase complex". Nat. Genet. 22 (4): 394–9. doi:10.1038/11970. PMID 10431247. S2CID 22326394.
• Maekawa M, Ishizaki T, Boku S, Watanabe N, Fujita A, Iwamatsu A, Obinata T, Ohashi K, Mizuno K, Narumiya S (1999). "Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase". Science. 285 (5429): 895–8. doi:10.1126/science.285.5429.895. PMID 10436159.
• Edwards DC, Sanders LC, Bokoch GM, Gill GN (1999). "Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics". Nat. Cell Biol. 1 (5): 253–9. doi:10.1038/12963. PMID 10559936. S2CID 25250183.
• Ohashi K, Nagata K, Maekawa M, Ishizaki T, Narumiya S, Mizuno K (2000). "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop". J. Biol. Chem. 275 (5): 3577–82. doi:10.1074/jbc.275.5.3577. PMID 10652353.
• Martindale DW, Wilson MD, Wang D, Burke RD, Chen X, Duronio V, Koop BF (2000). "Comparative genomic sequence analysis of the Williams syndrome region (LIMK1-RFC2) of human chromosome 7q11.23". Mamm. Genome. 11 (10): 890–8. doi:10.1007/s003350010166. PMID 11003705. S2CID 8575994.
• Sumi T, Matsumoto K, Shibuya A, Nakamura T (2001). "Activation of LIM kinases by myotonic dystrophy kinase-related Cdc42-binding kinase alpha". J. Biol. Chem. 276 (25): 23092–6. doi:10.1074/jbc.C100196200. PMID 11340065.
• Dan C, Kelly A, Bernard O, Minden A (2001). "Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin". J. Biol. Chem. 276 (34): 32115–21. doi:10.1074/jbc.M100871200. PMID 11413130.
• Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (2001). "Cofilin phosphorylation and actin reorganization activities of testicular protein kinase 2 and its predominant expression in testicular Sertoli cells". J. Biol. Chem. 276 (33): 31449–58. doi:10.1074/jbc.M102988200. PMID 11418599.

External links

• LIMK1 Info with links in the Cell Migration Gateway Archived 2014-12-11 at the Wayback Machine