The competition began on 28 November 2000 and ended with the final on 22 April 2001 at the Millennium Stadium.
In the first round, there were two sections: North and South. In the following rounds each section gradually eliminates teams in knock-out fashion until each has a winning finalist. At this point, the two winning finalists face each other in the combined final for the honour of the trophy. In addition to the 48 league teams, 8 Conference teams were also invited.