PPP1R12B

PPP1R12B
Identifiers
Aliases	PPP1R12B, MYPT2, PP1bp55, protein phosphatase 1 regulatory subunit 12B
External IDs	OMIM: 603768; MGI: 1916417; HomoloGene: 136757; GeneCards: PPP1R12B; OMA:PPP1R12B - orthologs
Gene location (Human) Chr. Chromosome 1 (human) Band 1q32.1 Start 202,348,699 bp End 202,592,706 bp
Gene location (Mouse) Chr. Chromosome 1 (mouse) Band 1|1 E4 Start 134,682,396 bp End 134,883,680 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in saphenous vein myocardium of left ventricle right coronary artery cardiac muscle tissue of right atrium right ventricle popliteal artery tibial arteries tail of epididymis thoracic aorta ascending aorta Top expressed in Rostral migratory stream ascending aorta muscle of thigh aortic valve soleus muscle extraocular muscle digastric muscle temporal muscle vastus lateralis muscle triceps brachii muscle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function enzyme activator activity protein binding protein kinase binding phosphatase regulator activity enzyme inhibitor activity Cellular component cytoplasm cytosol A band Z discdkac nucleoplasm cytoskeleton Biological process regulation of muscle contraction positive regulation of catalytic activity signal transduction regulation of catalytic activity negative regulation of catalytic activity G2/M transition of mitotic cell cycle Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 4660 329251 Ensembl ENSG00000077157 ENSMUSG00000073557 UniProt O60237 Q8BG95 RefSeq (mRNA) NM_001167857 NM_001167858 NM_001197131 NM_002481 NM_032103 NM_032104 NM_032105 NM_001331029 NM_001081307 NM_001368827 NM_001368829 RefSeq (protein) NP_001161329 NP_001161330 NP_001184060 NP_001317958 NP_002472 NP_115286 NP_115287 NP_001074776 NP_001355756 NP_001355758 Location (UCSC) Chr 1: 202.35 – 202.59 Mb Chr 1: 134.68 – 134.88 Mb PubMed search
Wikidata
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Protein phosphatase 1 regulatory subunit 12B is an enzyme that in humans is encoded by the PPP1R12B gene.

Myosin light chain phosphatase (MLCP) consists of three subunits- catalytic subunit, large subunit/myosin binding subunit (MBS) and small subunit (sm-M20). This gene is a multi-functional gene which encodes both MBS and sm-M20. MLCP regulates myosins and the dephosphorylation is enhanced by the presence of MBS. The sm-M20 is suggested to play a regulatory role in muscle contraction by binding to MBS. MBS is also encoded by another gene, myosin light chain phosphatase target subunit 1. sm-M20 shows higher binding affinity to this gene product than to myosin light chain phosphatase target subunit 2-MBS even though the two MBS proteins are highly similar. Although both MBSs increase the activity of MLCP, myosin light chain phosphatase target subunit 1-MBS is a more efficient activator. There are four alternatively spliced transcript variants described; two alter the MBS coding region and two alter the sm-M20 coding region of this gene.

Interactions

PPP1R12B has been shown to interact with Interleukin 16.

Further reading

• Somlyo AP, Wu X, Walker LA, Somlyo AV (1999). "Pharmacomechanical coupling: the role of calcium, G-proteins, kinases and phosphatases". Rev. Physiol. Biochem. Pharmacol. 134: 201–34. doi:10.1007/3-540-64753-8_5. ISBN 978-3-540-64753-9. PMID 10087910.
• Seki N, Ohira M, Nagase T, et al. (1998). "Characterization of cDNA clones in size-fractionated cDNA libraries from human brain". DNA Res. 4 (5): 345–9. doi:10.1093/dnares/4.5.345. PMID 9455484.
• Moorhead G, Johnson D, Morrice N, Cohen P (1998). "The major myosin phosphatase in skeletal muscle is a complex between the beta-isoform of protein phosphatase 1 and the MYPT2 gene product". FEBS Lett. 438 (3): 141–4. doi:10.1016/S0014-5793(98)01276-9. PMID 9827534. S2CID 35052936.
• Somlyo AP (1999). "Kinases, myosin phosphatase and Rho proteins: curiouser and curiouser". J. Physiol. 516 (3): 630. doi:10.1111/j.1469-7793.1999.0630u.x. PMC 2269296. PMID 10200412.
• Arimura T, Suematsu N, Zhou YB, et al. (2001). "Identification, characterization, and functional analysis of heart-specific myosin light chain phosphatase small subunit". J. Biol. Chem. 276 (9): 6073–82. doi:10.1074/jbc.M008566200. PMID 11067852.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Bannert N, Vollhardt K, Asomuddinov B, et al. (2004). "PDZ Domain-mediated interaction of interleukin-16 precursor proteins with myosin phosphatase targeting subunits". J. Biol. Chem. 278 (43): 42190–9. doi:10.1074/jbc.M306669200. PMID 12923170.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
• Okamoto R, Kato T, Mizoguchi A, et al. (2007). "Characterization and function of MYPT2, a target subunit of myosin phosphatase in heart". Cell. Signal. 18 (9): 1408–16. doi:10.1016/j.cellsig.2005.11.001. PMID 16431080.
• Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315–21. Bibcode:2006Natur.441..315G. doi:10.1038/nature04727. PMID 16710414.